Thermo Scientific™ Pierce™ Magnetic ChIP Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI26157-Each-of-1 | In Stock | ₹ 83,035.98 |
PI26157 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 83,035.98
GST (18%): ₹ 14,946.476
Total Price: ₹ 97,982.456
Capacity (Metric)
4 x 106cells/reaction
Content And Storage
• Glycine Solution (10X), 15 mL; store at room temperature• PBS (20X), 15 mL; store at 4°C• Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X), 1 mL; store at 4°C• Membrane Extraction Buffer, 10 mL; store at 4°C• MNase Digestion Buffer, 10 mL; store at 4°C• DTT (7.7 mg), Lyophilized, 2 vials; store at room temperature or 4°C• MNase Stop Solution, 1 mL; store at 4°C• ChIP-Grade Protein A/G Magnetic Beads, 0.7mL; store at 4°C• IP Dilution/Wash Buffer (5X), 40 mL, store at 4°C• Sodium Chloride (5M), 6 mL; store at 4°C• IP Elution Buffer (2X), 4.5 mL; store at 4°C• Microcentrifuge Tubes, 1.5 mL, 75 tubes; store at room temperature or 4°C• DNA Clean-Up Column and Reagents, 40 purifications; store at room temperature or 4°C• DNA Clean-Up Column, 40 columns• DNA Column Binding Solution, 30 mL• DNA Column Wash Solution, 6 mL• pH Indicator, 0.8 mL• DNA Column Elution Solution, 5 mL• Micrococcal Nuclease (ChIP Grade) (10U/μL), 50 μL; store at -20°C• Anti-RNA Polymerase II Antibody, 25 μL; store at -20°C• Normal Rabbit IgG (1mg/mL), 10 μL; store at -20°C• Proteinase K (20mg/mL), 0.25 mL; store at -20°C• ChIP Positive Control Primers (GAPDH promoter, Human-specific), 100 μL; store at -20°C
Quantity
30 reactions
Sufficient For
30 Reactions
Description
Pierce Magnetic ChIP Kit
Product Line
Pierce™
Type
ChIP Kit
Format
Kit
Description
- Efficiently isolate chromatin-bound DNA by immunoprecipitation (chromatin IP) for subsequent quantitation by PCR using Thermo Scientific™ Pierce Magnetic ChIP Kit
- Additional Information and Online Ordering The Pierce Magnetic ChIP Kit contains sufficient reagents to perform 30 chromatin immunoprecipitation (ChIP) assays with appropriate controls using an optimized protocol
- The kit includes reagents for cell lysis, capture of protein-DNA complexes, reversal of crosslinking, and DNA isolation
- The blocked Pierce Protein A/G Magnetic Beads provide high binding capacity, low non-specific background, and work with many antibody species
- These beads can be used manually with a magnetic stand or with automated platforms, such as the Thermo Scientific KingFisher Instruments
- ChIP-validated and quality-guaranteed antibodies are also available for use with the Pierce Magnetic ChIP Kit
- Highlights: Rapid – obtain purified DNA that is ready for quantitative PCR in about 8 hours Efficient and reproducible – micrococcal nuclease digestion and nuclear lysis are highly optimized Sensitive – obtain results with as few as 10,000 cells (1 x 10 4 ) Low background – Pierce Protein A/G Magnetic Beads are blocked in a non-DNA-containing reagent to minimize background Complete – optimized positive control reagents are included: RNA polymerase II antibody and GAPDH promoter PCR primers Includes: Kit contains reagents for chromatin preparation, IP, DNA purification, and positive controls (antibody and primers) Requires: In vivo crosslinker (such as formaldehyde), micro-tip sonicator (such as Misonix™ Sonicator 3000), ChIP qualified antibody of choice, PCR primers for DNA sequence of interest, PCR master mix (containing a dye such as SYBR™ Green, if qPCR is desired), and a qPCR instrument Recommended for: Determine sites of specific protein-DNA interactions on genomic DNA by PCR or ChIP-Seq Monitor the effects of histone modifications or chemical agents on DNA binding
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Capacity (Metric): 4 x 106cells/reaction
Content And Storage: • Glycine Solution (10X), 15 mL; store at room temperature• PBS (20X), 15 mL; store at 4°C• Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X), 1 mL; store at 4°C• Membrane Extraction Buffer, 10 mL; store at 4°C• MNase Digestion Buffer, 10 mL; store at 4°C• DTT (7.7 mg), Lyophilized, 2 vials; store at room temperature or 4°C• MNase Stop Solution, 1 mL; store at 4°C• ChIP-Grade Protein A/G Magnetic Beads, 0.7mL; store at 4°C• IP Dilution/Wash Buffer (5X), 40 mL, store at 4°C• Sodium Chloride (5M), 6 mL; store at 4°C• IP Elution Buffer (2X), 4.5 mL; store at 4°C• Microcentrifuge Tubes, 1.5 mL, 75 tubes; store at room temperature or 4°C• DNA Clean-Up Column and Reagents, 40 purifications; store at room temperature or 4°C• DNA Clean-Up Column, 40 columns• DNA Column Binding Solution, 30 mL• DNA Column Wash Solution, 6 mL• pH Indicator, 0.8 mL• DNA Column Elution Solution, 5 mL• Micrococcal Nuclease (ChIP Grade) (10U/μL), 50 μL; store at -20°C• Anti-RNA Polymerase II Antibody, 25 μL; store at -20°C• Normal Rabbit IgG (1mg/mL), 10 μL; store at -20°C• Proteinase K (20mg/mL), 0.25 mL; store at -20°C• ChIP Positive Control Primers (GAPDH promoter, Human-specific), 100 μL; store at -20°C
Quantity: 30 reactions
Sufficient For: 30 Reactions
Description: Pierce Magnetic ChIP Kit
Product Line: Pierce™
Type: ChIP Kit
Format: Kit
Capacity (Metric): ≥50 mg Human IgG per mL Resin Binding
Content And Storage: Upon receipt store at 4°C
Quantity: 0.65 mL
Sufficient For: __
Description: __
Product Line: Pierce™
Type: Agarose
Format: Bottle
Capacity (Metric): __
Content And Storage: Upon receipt store at -20°C.
Quantity: 0.25 mL
Sufficient For: __
Description: Pierce Proteinase K
Product Line: __
Type: Proteinase K (ChIP grade)
Format: __
