Thermo Scientific™ CHAPS Detergent (3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate)

Description

• Thermo Scientific™ CHAPS is a zwitterionic detergent that is especially well suited for protecting the native state of proteins
• CHAPS is a sulfobetaine derivatives of cholic acid
• This zwitterionic detergent is useful for membrane protein solubilization when it is important to maintain protein activity
• CHAPS detergent is soluble over a wide range of pH (2 to 12) and are easily removed from solution by dialysis because of their high critical micelle concentration (CMC)
• Highlights: Versatile zwitterionic detergents Provide mild but effective lysis of cultured mammalian cells Nondenaturing and generally do not inactivate protein functions Can be removed from solution by dialysis Commonly used for isoelectric focusing (IEF) and 2D electrophoresis Properties of CHAPS detergent: Chemical Name: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate Molecular Weight: 614.88 Detergent Class: Zwitterionic Aggregation Number: 10 Micelle Molecular Weight: 6149 Critical Micelle Concentration (CMC): 8 to 10mM (0.4920 to 0.6150%, w/v) Cloud Point: ≥ 100°C Dialyzable: Yes