Thermo Scientific™ Inclusion Body Solubilization Reagent

Description

• Effectively retrieve recombinant proteins that form insoluble aggregates during bacterial overexpression with Thermo Scientific™ Inclusion Body Solubilization Reagent
• The Inclusion Body Solubilization Reagent is a ready-to-use aqueous solution of denaturant that helps to dissolve aggregated proteins (inclusion bodies) that have been separated from the soluble fraction of bacterial cell lysates
• The reagent is designed to retrieve expressed protein in soluble form after lysis and extraction with reagents such as Thermo Scientific B-PER Bacterial Protein Extraction Reagent
• Bacteria are widely used for recombinant protein expression
• However, 70%-80% of proteins produced by recombinant techniques in E
• coli form inclusion bodies (i.e., protein aggregates)
• Once these aggregates are formed, it is very difficult to solubilize them
• A proprietary denaturant contained in this reagent provides the most effective means for solubilizing aggregated proteins
• Additional components, such as a reducing agent and a chelating agent, may be added to the reagent, depending on the particular application
• Highlights: Achieves the essential first step for subsequent insoluble protein refolding procedures One easy- and ready-to-use solution; no preparation needed Compatible with Thermo Scientific Coomassie Plus Protein Assay Kit (Part No
• 23236) to determine degree of expression Proprietary denaturant can be easily removed by dialysis so that SDS-PAGE can be performed Can accommodate additional components such as reducing agents or chelating agents Compatible with: Thermo Scientific Pierce Protein Refolding Kit (Part No
• 89867)