Thermo Scientific™ Pierce™ Protein Refolding Kit

Description

• Thermo Scientific™ Pierce Protein Refolding Kit includes high-purity reagents and detailed instructions for using a matrix strategy to determine optimal buffer conditions for refolding recombinant proteins that have been denatured and solubilized from inclusion bodies
• The kit contains the essential reagents and complete strategy for determining optimal buffer conditions to refold denatured recombinant proteins to restore native structure and function
• Nine base refolding buffers form a matrix that includes a range of strong and weak denaturant conditions for the suppression of protein aggregation
• The supplied additives are used as additional matrix factors, depending on the protein type being refolded
• Buffer components are examined at three concentration levels, allowing a wide spectrum of folding conditions to be tested within one experiment
• The adjustable design allows matrix conditions to be tailored to the target protein, preventing sample waste and unnecessary analysis, while maximizing refolding yields
• The Pierce Protein Refolding Kit is accompanied by a comprehensive Refolding Guide with details on isolating, solubilizing and purifying inclusion bodies; optimizing refolding conditions; and analyzing refolding yields
• Highlights: Robust – conditions and components examined are limited to those having the most significant and general utility as folding buffers Convenient – three-level matrix design significantly reduces the amount of secondary optimization required and increases the ease of data interpretation Adjustable matrix format – allows refolding experiments to be customized to the target protein; known positive and negative interactions between buffer components are addressed, minimizing unnecessary analyses High-purity reagents – reagents are formulated using stringent standards so that consistent results are attained Includes: Nine different Base Refolding Buffers (10mL each), convenient amounts of seven additives, and a comprehensive Refolding Guidebook Compatible with: Thermo Scientific Inclusion Body Solubilization Reagent (Part No
• 78115)