Thermo Scientific™ Pierce™ Protein A Magnetic Beads

Description

• Perform antibody purification and immunoprecipitation methods using manual or robotic magnetic separators with Thermo Scientific™ Pierce Protein A Magnetic Beads, high-capacity and high-throughput affinity particles
• Additional Information and Online Ordering Recombinant protein A, covalently attached to a blocked magnetic bead surface, can bind to antibodies from many different species, enabling purification of antibodies from crude extracts
• Immunoprecipitation assays performed with protein A coated beads result in high yield of target antigen with very low background
• Using our crosslinker chemistry, you can immobilize an antibody onto the magnetic particle and prevent IgG contamination in IP or Co-IP experiments
• The Pierce Protein A Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms, such as the Thermo Scientific™ KingFisher Instruments, for high-throughput workflows
• Highlights: High efficiency – equivalent or higher yield of IP target antigens than magnetic beads from other suppliers Low non-specific binding – stable, pre-blocked beads provide highly purified product (e.g., antigen eluted in IP with antibody is devoid of contaminating protein from complex cell lysate) Consistent – magnetic beads eliminate resin loss and provide for more efficient separation than traditional IP methods that use only centrifugation Versatile – beads are compatible with manual and automated workflows (e.g., Thermo Scientific™KingFisher Instruments) Recommended for: IP and Co-IP experiments Antibody purification