Thermo Scientific™ Pierce™ Protein A/G Magnetic Beads

Description

• The Thermo Scientific™ Pierce Protein A/G Magnetic Beads are high-performance affinity particles for antibody purification and immunoprecipitation methods using manual or robotic magnetic separators
• These magnetic beads are coated with genetically engineered Pierce Protein A/G, a recombinant fusion protein which combines the IgG binding domains of both Protein A and Protein G
• This enables capture of antibodies from a wider range of species and isotypes than either protein alone
• Using our crosslinker chemistry, you can immobilize an antibody onto the magnetic particle and prevent IgG contamination in your immunoprecipitated sample
• These beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific KingFisher™ Instruments
• Highlights: High capacity – nearly four times higher binding capacity than typical magnetic beads from other suppliers, allowing the use of smaller amounts per experiment Low non-specific binding – stable, pre-blocked beads provide clean purification products (e.g., antigen eluted in IP with antibody is devoid of contaminating proteins from complex IP matrix) Flexibility – convenience of IgG binding domains of both Protein A and Protein G on one bead Compatibility - beads are compatible with manual and automated applications (e.g., Thermo Scientific KingFisher Instruments) Assay consistency – magnetic beads eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only microcentrifuge tubes Properties of Thermo Scientific Pierce Protein A/G Magnetic Beads: Composition: Magnetite-coated polymeric beads blocked and covalently coated with a monolayer of recombinant Protein A/G Diameter: 1μm Concentration: 10mg/mL Binding Capacity: 55-85μg rabbit IgG per mg magnetic particles Requires: Thermo Scientific KingFisher 96 or KingFisher Flex Instrument or other magnetic separator device Recommended for: IP and Co-IP experiments (see complete kit); Immunoprecipitation for analysis in non-reducing conditions; Antibody purification