Thermo Scientific™ Pierce™ NHS-Activated Magnetic Beads

Description

• Thermo Scientific™ Pierce NHS-Activated Magnetic Beads enable covalent, amine-based conjugation of proteins to magnetic beads in a simple mix-and-go format for use in custom affinity purification experiments
• The activated magnetic beads contain N -hydroxysuccinimide (NHS) functional groups that react with primary amines forming stable amide linkages
• Once they are covalently attached, the immobilized proteins are highly resistant to leaching from the bead surface
• When prepared beads are used in experiments, nonspecific binding is negligible because nonreacted NHS-ester groups are thoroughly blocked during the coupling procedure
• Pierce NHS-Activated Magnetic beads can be coupled and processed either manually with a magnetic stand or with automated platforms such as the Thermo Scientific KingFisher Instruments
• Highlights: High capacity at least four times higher binding capacity than NHS-activated magnetic beads from other suppliers Easy to use immobilize in a simple one-step reaction with minimal hands-on time Safe no hazardous chemicals needed (e.g., sodium cyanoborohydride and cyanogen bromide) Ligand-compatible use to immobilize with nearly any primary amine-containing compound or affinity ligand Low non-specific binding the bead surface is pre-blocked and any nonreacted NHS-ester groups are fully quenched Protocol-compatible protein coupling to the beads and downstream applications can be performed manually or by automation (e.g., Thermo Scientific KingFisher Instruments) Recommended for: Immobilization of ligands for the purification of recombinant proteins; Immobilization of antibodies for immunoprecipitations and co-immunoprecipitations free of antibody contamination in the eluates; Immobilization of secondary antibody for the affinity purification of antibody subtypes from ascites, serum and cell culture supernatant