Thermo Scientific™ Pierce™ Protein L Magnetic Beads

Description

• Perform antibody purification using manual or robotic magnetic separators with Thermo Scientific™ Pierce Protein L Magnetic Beads, high-capacity and high-throughput affinity particles
• Additional Information and Online Ordering Recombinant Protein L, covalently attached to a blocked magnetic bead surface, selectively binds mouse and human antibodies through kappa light chains
• The beads are commonly used to purify monoclonal antibodies in cell culture supernantants supplemented with bovine serum as Protein L does not bind bovine IgG
• The Pierce Protein L Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms such as the Thermo Scientific™ KingFisher Instruments for high-throughput workflows
• Highlights: Selective – immobilized Protein L is ideal for selective purification of human and mouse antibodies that have kappa light chains Low non-specific binding – stable, pre-blocked beads provide clean purification of antibody Versatile – beads are compatible with manual and automated workflows (e.g., Thermo Scientific™ KingFisher Instruments) Recommended for: Purification of monoclonal and polyclonal antibodies that bind poorly to Protein A or Protein G magnetic beads Purification of monoclonal antibodies from culture supernatants supplemented with bovine serum Protein L IP and co-IP assays Purification of ScFv and Fab fragments containing kappa light chains