Thermo Scientific™ Pierce™ Cell Surface Protein Isolation Kit

Description

• Thermo Scientific™ Cell Surface Protein Isolation Kit is a complete set of reagents for selective biotinylation and subsequent purification of mammalian cell surface proteins to the exclusion of intracellular proteins
• The kit efficiently labels proteins that have accessible lysine residues and sufficient extracellular exposure.The isolation procedure uses a cell-impermeable, cleavable biotinylation reagent (Sulfo-NHS-SS-Biotin) to label exposed primary amines of proteins on the surface of intact adherent or suspension cells
• Treated cells are then harvested and lysed, and the labeled surface proteins are affinity-purified using Thermo Scientific NeutrAvidin Agarose Resin
• The isolated cell surface proteins contain a small, nonreactive tag of the originally labeled primary amines but are no longer biotinylated (biotin remains bound to the resin)
• The kit contains sufficient reagents for eight experiments, each involving four T75 flasks of confluent cells
• Highlights: Isolates cell surface proteins – reduces complexity of total cellular protein Efficiently recovers labeled proteins – cleavable biotin allows for nearly 100% recovery of isolated cell surface proteins Convenience – all reagents are provided in one kit, along with complete instructions for labeling, cell lysis and purification of cell surface membrane proteins Western blotting applications – proteins recovered in SDS-PAGE buffer are loaded directly onto polyacrylamide gels Robust system – protocol designed for diverse cell lines, including NIH 3T3, HeLa, C6 and A431 Includes: Biotinylation reagent, cell lysis reagent, agarose affinity resin, various buffers and microcentrifuge spin columns