Thermo Scientific™ Mem-PER™ Plus Membrane Protein Extraction Kit

Description

• Perform small-scale solubilization and enrichment of integral membrane proteins and membrane-associated proteins in a simple reagent-based procedure using Thermo Scientific™ Mem-PER Plus Membrane Protein Extraction Kit
• Additional Information and Online Ordering The Mem-PER Plus Kit effectively isolates membrane proteins from cultured cells and tissues using a simple benchtop microcentrifuge procedure
• Integral membrane proteins having one or two membrane-spanning domains are effectively solubilized and isolated from cytosolic proteins
• The kit makes possible the extraction and selective enrichment of integral and attached membrane proteins from cultured mammalian cells, as well as from hard and soft mammalian tissues
• The Mem-PER Plus Kit has many advantages compared to our original Mem-PER Kit
• The protocol is simpler, and the fractions are directly compatible with many downstream applications such as SDS-PAGE, Western Blotting, BCA, immunoprecipitation, and amine-reactive protein labeling techniques
• Highlights: Extraction and isolation – produces minimal cross-contamination (typically less than 10%) of cytosolic protein into the membrane protein fraction Cells or tissues – effective for extraction from cultured mammalian cells and mammalian tissues Downstream compatibility – analyze membrane protein extracts by SDS-PAGE, Western blotting, immunoprecipitation and protein assays Fast and simple – isolation of membrane proteins in approximately one hour No special equipment required – only a benchtop microcentrifuge, tubes, homogenizers, and pipettors are needed Includes: Kit contains a cell wash solution, permeabilization buffer, and solubilization buffer.