Thermo Scientific™ Subcellular Protein Fractionation Kit for Tissues

Description

• The Thermo Scientific™ Subcellular Protein Fractionation Kit for Tissue includes all reagents and tissue strainers required to perform cell fractionation and enrichment of proteins from five different cellular compartments within 2 hours
• The complete Subcellular Protein Fractionation Kit for Tissue includes a unique 250μm tissue strainer to facilitate rapid separation of cell suspension from tissue debris after homogenization
• The optimized reagents enable subsequent stepwise extraction of cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins from the recovered cells with less than 15% cross-contamination
• Tissue extracts obtained with this subcellular fractionation kit are compatible with a variety of downstream applications, including Western blotting, protein quantitation, electrophoretic mobility shift, reporter-gene and enzyme-activity assays
• Highlights: Optimized formulations and protocols specific for fractionation of tissue Complete obtain cytoplasmic, membrane, soluble nuclear, chromatin-bound and cytoskeletal protein fractions from a single kit Time efficient extract functional protein fractions without ultracentrifugation in approximately 2 hours Convenient unique tissue strainer allows rapid removal of tissue debris Compatible use extracts for downstream applications such as protein assays, Western blotting, gel-shift assays and enzyme-activity assays Versatile extract proteins from a variety of tissue types, including liver, heart, brain, kidney, lung and spleen Recommended for: Obtain cytoplasmic, soluble nuclear, chromatin-bound, membrane and cytoskeletal proteins from the same tissue sample; Profile disease-state versus normal tissues for proteins from the cytoplasm, nucleus, membrane and cytoskeleton; Study protein translocation in tissues