Thermo Scientific™ Mitochondria Isolation Kit for Cultured Cells

Description

• Thermo Scientific™ Mitochondria Isolation Kit for Cultured Cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples in about 40 minutes
• Isolation of mitochondria is typically a laborious process requiring single-sample processing with Dounce homogenization
• The Mitochondria Isolation Kit uses a non-mechanical, reagent-based method that allows multiple (six) cultured cell samples to be processed concurrently in about 40 minutes
• Cultured mammalian cell pellets are gently lysed using a proprietary formulation that results in maximum yield of mitochondria with minimal damage to integrity
• The product instructions describe guidelines for optimizing purity vs
• yield parameters
• The kit also offers an optimized Dounce homogenization procedure, which results in two-fold greater mitochondria recovery compared to the reagent-based method
• Both methods use differential centrifugation to separate the mitochondrial and cytosolic fractions with a benchtop microcentrifuge and are completed in approximately 40 minutes (post-cell harvest)
• Once isolated, the mitochondria can be used in downstream applications such as apoptosis, signal transduction and metabolic studies, as well as to facilitate mitochondrial proteomics efforts
• Highlights: Fast – isolate intact mitochondria from 2 × 10 7 cells in approximately 40 minutes (post-cell harvest) Flexible – process several samples simultaneously using the reagent-based method, or obtain highest possible yield with the Dounce homogenization method Optimizable – guidelines provided for optimizing purity vs
• yield with the reagent-based method Benchtop-easy – isolation performed in a microcentrifuge tube Compatible – isolated mitochondria may be lysed with detergent or processed for almost any downstream application, including 2D electrophoresis Includes: Three buffer and detergent solutions totaling approx
• 115mL