Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads

Description

• Accelerate throughput for automated magnetic purification of biotinylated molecules with Thermo Scientific™ Pierce Streptavidin Magnetic Beads
• Magnetic streptavidin beads enable affinity purification of biotin-labeled target molecules without columns or centrifugation
• The platform provides a fast and convenient method for manual or automated immunoprecipitation, protein interaction analysis, DNA-protein pull-down, and purification involving biotin-labeled proteins and nucleic acids
• The iron oxide superparamagnetic particles offer superior performance (high capacity and low nonspecific binding) compared with other commercial magnetic beads
• Biotinylated molecules are simply added to the streptavidin-coated magnetic beads for binding
• Pierce Streptavidin Magnetic Beads are validated and optimized for use with high-throughput magnetic platforms, such as the Thermo Scientific KingFisher™ 96 and KingFisher Flex Instruments, but the beads also enable premium performance for simple benchtop applications using an appropriate magnetic stand
• Highlights: High-performance beads – non-aggregating, pre-blocked, iron oxide, superparamagnetic microparticles provide exceptional uniformity for automated HTS and manual applications alike Stable immobilization chemistry – streptavidin is immobilized using leach-resistant chemistry High capacity – superior quality beads with high binding capacity provide rapid and efficient biomolecule purification from complex samples Low non-specific binding – stable, pre-blocked beads provide clean purification products (e.g., antigen eluted in IP with biotinylated antibody) that are compatible with mass spectrometry analysis and other downstream analyses Superior performance – nearly three times higher binding capacity than typical beads from other suppliers, allowing the use of smaller amounts per experiment Properties of Streptavidin Magnetic Beads: Composition: ion oxide particles covalently coated with a monolayer of streptavidin protein Magnetization: superparamagnetic (no magnetic memory) Bead size: 1μm (nominal mean diameter) Density: 2g/cm 3 Concentration: 10mg beads per milliliter of slurry in 0.05% sodium azide Binding capacity: approx
• 55μg biotinylated rabbit IgG per mg of beads; approx
• 3500pmol biotinylated fluorescein per milliliter of beads RNase: these bead solutions are NOT tested and certified to be RNAse-free Requires: Thermo Scientific KingFisher 96 or KingFisher Flex Instrument or other magnetic separator device Recommended for: Immunoprecipitate antigens (using biotinylated antibodies) from a wide variety of sources; Co-immunoprecipitate interaction complexes using biotinylated antibodies; Capture protein-protein interactions in pull-down assays using biotinylated bait proteins; Isolate biotin-labeled DNA-protein complexes from cell or tissue extracts; Capture single-stranded biotinylated DNA oligos; Isolate biotinylated PCR products