Thermo Scientific™ ActivX™ TAMRA-FP Serine Hydrolase Probe

Description

• The TAMRA-FP probe feature a reactive fluorophosphonate group that is used to label and detect active serine hydrolases by fluorescent gel imaging, capillary electrophoresis or mass spectrometry
• An anti-TAMRA antibody is also available for immuno-enrichment of TAMRA-FP probe-labeled proteins
• ActivX™ active-site enrichment probes are advantageous for determining active enzyme levels through the use of analog-tagged probes to allow profiling of both inactive and active enzymes
• The ActivX probes selectively enrich only those enzymes that are functionally active and biologically relevant at the time of labeling
• With selective enrichment, one can identify and profile target enzyme classes across samples or can assess the specificity and affinity of enzyme inhibitors
• The serine hydrolase superfamily is one of the largest, most diverse enzyme families in eukaryotic proteomes
• Serine hydrolases are generally grouped into two large families: serine proteases (e.g., trypsin, elastase and thrombin) and metabolic serine hydrolases
• Metabolic serine hydrolases are divided into multiple enzyme subclasses (e.g., esterases, lipases, amidases and peptidases) based on structure, catalytic mechanism and substrate preference
• These probes can be used to assess activity or screen small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins
• These products are subject to a limited use label license.