Thermo Scientific™ HisPur™ Ni-NTA Magnetic Beads

Description

• Perform affinity purification of His-tagged fusion proteins in manual or automated formats with Thermo Scientific™ HisPur Ni-NTA Magnetic Beads, high capacity nickel-IMAC beads
• Additional Information and Online Ordering The blocked magnetic bead surface is derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+)
• The immobilized metal affinity chromatography (IMAC) beads provide high binding capacity with very low background
• The HisPur Ni–NTA Magnetic Beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific™ KingFisher Instruments for high–throughput needs
• Highlights: High capacity – equivalent or higher binding capacity than Ni-NTA magnetic beads from other suppliers Low nonspecific binding – the bead surface is pre-blocked and the protocol provides optimized buffers for purification Fast – protocol is completed in 1 hour Scalable – process microliter to milliliter sample volumes Versatile – purify proteins using native or denaturing conditions Reagent compatible – can be used with common cell lysis reagents and a variety of buffer additives Multiple formats – protein coupling to the beads and downstream applications can be performed both manually and on an automated platform (e.g., Thermo Scientific™ KingFisher Instruments)