Thermo Scientific™ Pierce™ Anti-DYKDDDDK Magnetic Agarose

Description

• Thermo Scientific Pierce Anti-DYKDDDDK Magnetic Agarose provides a fast, convenient method for purification and immunoprecipitation (IP) of DYKDDDDK-tagged proteins from in vitro protein expression systems, bacteria, yeast, and mammalian cells
• The amino acid sequence DYKDDDDK, commonly known as “FLAG”, is recognized by a high-affinity rat monoclonal antibody (clone L5) that is covalently attached to a magnetite-embedded agarose core particle
• For protein purification, the magnetic agarose is added to a sample containing DYKDDDDK-tagged protein(s) with the tag on either the N- or the C-terminus
• Captured proteins are then magnetically separated from the supernatant, and non-specifically bound proteins can be washed away before dissociating bound DYKDDDDK-tagged protein(s) with elution buffer
• The magnetic agarose is removed from the solution using a magnetic stand or an instrument such as the KingFisher Flex Magnetic Particle Processor
• Automated instruments are especially useful for higher throughput purifications and screening of purification conditions
• Specific—Unique base-beads and highly specific antibody minimizes off-target binding (low non-specific binding) High purity —Optimized Bind-Wash-Elute protocol enables high purity High Yield —special antibody conjugation method enables high yield Rapid—entire purification protocol typically takes less than 40 mins Economical - purification protocol allows multiple reuses Versatile—beads are compatible with manual and automated workflows (e.g., KingFisher instruments)