Thermo Scientific™ Pierce™ Anti-DYKDDDDK Affinity Resin

Description

• The Thermo Scientific Pierce Anti-DYKDDDDK Affinity Resin provides a fast, convenient method for purification and immunoprecipitation (IP) of DYKDDDDK-tagged proteins expressed in in vitro protein expression systems, bacteria, yeast, and mammalian cells
• The amino acid sequence DYKDDDDK, commonly known as “Flag”, is recognized by a high-affinity rat monoclonal antibody (clone L5) that is covalently attached to UltraLink Biosupport
• For protein purification or IP, resin is added to a sample containing DYKDDDDK-tagged protein(s) with the tag on either the N- or the C-terminus
• Following capture of DYKDDDDK-tagged proteins, non-specifically bound proteins can be washed away before dissociating bound target protein(s) with elution buffer
• The Pierce Anti-DYKDDDDK Affinity Resin is recommended for use in spin purification columns or cartridges for FPLC instruments
• UltraLink Biosupport is a rigid, hydrophilic, highly crosslinked, copolymeric and porous resin with high coupling capacity
• The porosity, rigidity, and durability of the resin also make it suitable for medium-pressure, fast-flow techniques involving large sample volumes
• Specific—Unique base-beads and highly specific antibody minimizes off-target binding (low non-specific binding) High purity —Optimized Bind-Wash-Elute protocol enables high purity High Yield —special antibody conjugation method enables high yield Rapid—entire purification protocol typically takes less than 40 mins Economical—purification protocol allows multiple reuses Versatile—can be used in spin-, gravity- or FPLC-based purification and enrichment methods Scalable—available in 1, 5, and 50 mL settled resin package sizes to allow for smaller scale immunoprecipitations or larger scale purifications