NanoLuc™-HaloTag™ fusion gene Kanamycin resistance gene for selection in bacteria A CMV promoter for high-level expression of NanoLuc™-HaloTag™ fusion in mammalian cells The NanoBRET™ Positive Control Vector should be diluted in Transfection Carrier DNA to reduce cellular expression levels due to the high NanoBRET™ signal produced
The control vector can be used when performing instrument setup or as a reference control when performing other NanoBRET™ assays.