The p53, MDM2 assay gives a strong NanoBRET™ signal and can be used when performing instrument setup or included as a reference control pair when performing other NanoBRET™ PPI assays
The p53-HaloTag™ Fusion Vector contains the following key features: p53-HaloTag™ fusion gene Kanamycin resistance gene for selection in bacteria A CMV promoter for high-level expression of the p53-HaloTag™ fusion in mammalian cells The NanoLuc™-MDM2 Fusion Vector contains the following key features: NanoLuc™-MDM2 fusion gene Kanamycin resistance gene for selection in bacteria A CMV promoter for high-level expression of the NanoLuc™-MDM2 fusion in mammalian cells