3667293
Cytiva HiTrap™ rProtein A FF Columns
Use for preparative purification of monoclonal and polyclonal antibodies from cell culture supernatants, serum and ascites. Cytiva Life Sciences™ HiTrap™ rProtein A FF Columns are prepacked with rProtein A Sepharose™ Fast Flow.
Manufacturer: Fischer Scientific
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Binding Capacity
≈50 mg Human IgG/mL
For Use With (Application)
Chromatography System, Manual
For Use With (Equipment)
For syringe, peristaltic pump or a chromatography system
Content And Storage
4°C to 30°C, 20% Ethanol
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Description
- Packed with rProtein A Sepharose™ Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources Exhibits similar Fc region specificity to that of native protein A but with enhanced binding capacity and fewer regulatory concerns due to total absence of mammalian culture in ligand production and purification Average particle size: 90μm Designed for use with a syringe, a peristaltic pump, or a chromatography system Available as 1mL and 5mL columns Media Ligand: Recombinant protein A ( E.coli ) Matrix: Highly cross-linked 4% agarose Binding capacity: ∽50mg human IgG/mL pH stability working range: 3 to 10 1mL columns Bed dimensions: 7 x 25mm Flow velocity: <4mL/min
- 5mL columns Bed dimensions: 16 x 25mm Flow velocity: <20mL/min
- Order Info 1mL and 5mL columns are packed with 1mL and 5mL of rProtein A Sepharose Fast Flow, respectively.
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Binding Capacity: ≈50 mg Human IgG/mL
For Use With (Application): Chromatography System, Manual
For Use With (Equipment): For syringe, peristaltic pump or a chromatography system
Content And Storage: 4°C to 30°C, 20% Ethanol
Binding Capacity:
≈50 mg Human IgG/mL
For Use With (Application):
Chromatography System, Manual
For Use With (Equipment):
For syringe, peristaltic pump or a chromatography system
Content And Storage:
4°C to 30°C, 20% Ethanol
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