Thermo Scientific™ Pierce™ Biotin

Description

• Thermo Scientific™ Pierce Biotinylated Proteins include biotin-labeled horseradish peroxidase (B-HRP), alkaline phosphatase (B-AP) and fluorescein (B-FITC) for use as controls or signal amplification in IHC via avidin-biotin complex (ABC) techniques
• Biotinylated HRP and biotinylated AP are most commonly used in immunohistochemistry (IHC) to amplify the signal of biotinylated primary antibodies using the ABC staining method
• Biotinylated Fluorescein (FITC) cannot be used to polymerize and amplify the signal to the same degree as biotinylated enzymes because each fluor molecule is tagged with only one biotin molecule
• However, the fluorescent variant of traditional ABC staining is possible with this unique molecule
• Highlights: Biotinylated enzymes – biotin-labeled horseradish peroxidase (B-HRP), alkaline phosphatase (B-AP) and beta-galactosidase (B-bGal) for use in avidin-biotin complex (ABC) staining Biotinylated FITC – amplify the fluorescein signal of biotinylated primary antibodies by using avidin or streptavidin plus biotinylated fluorescein (B-FITC) Biotinylated controls – use biotinylated bovine serum albumin (B-BSA) or any one of these molecules as positive controls in biotin quantitation methods