Anti-FOXO1 Antibody, clone 2H8.2

Description

• General description: Forkhead box O (FOXO) transcription factors play an important role in modulating metabolic functions. Given the relatively high expression of FOXO1 in insulin-responsive tissues, this transcription factor is highly poised to regulate energy metabolism. When nutrient and insulin levels are low, FOXO1 promotes expression of gluconeogenic enzymes. Conversely, in the fed state, insulin levels rise and stimulate uptake of glucose primarily into skeletal muscle and other organs, including adipose tissue. Under certain pathophysiologic conditions, including insulin resistance, negative signaling to FOXO1 is compromised. (Gross, D.N., 2009).
• Specificity: This antibody detects FOXO1. FOXO1 shuttles between the nucleus and the cytosol.
• Immunogen: GST-tagged recombinant protein corresponding to full length FOXO1.
• Application: Immunocytochemistry: A 1:500 dilution of a previous lot was used. Immunocytochemistry: Representative lot data.A 1:500 dilution of a previous lot was used. Immunohistochemistry (Paraffin): Representative lot data.Optimal Staining With Epitope Retrieval: Ductal Carcinoma In Situ.
• Quality: Evaluated by western blot on HEK293 cell lysate.Western Blot Analysis: 2 µg/mL of this antibody detected FOXO1 in HEK293 cell lysate.
• Target description: ~78-82 kDa
• Linkage: Replaces: 04-1005
• Physical form: Format: Purified
• Storage and Stability: Stable for 1 year at 2-8ºC from date of receipt.
• Analysis Note: ControlHEK293 cell lysate
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.