Anti-β-Catenin Antibody

Description

• General description: β-catenin plays a central role in cell adhesion, bridging between cadherins and actin cytoskeleton. These activities are regulated through its differential interaction with several molecular partners, including cell-cell adherens junction (AJ) molecules, components of its degradation complex, and transcription factors. β-Catenin is composed of three domains: a central armadillo repeat domain and two N- and C-terminal tails. β-catenin can be phosphorylated by serine-threonine kinase, such as GSK-3. The phosphorylated β-catenin affects the protein association of β-catenin to E-cadherin and α-cateinin that occurs in the cell. This process can be reversed by de-phosphorylation of β-catenin.
• Specificity: Recognizes β-Catenin, Mr 92 kDa.
• Immunogen: KLH-conjugated, synthetic peptide (CGG-SYLDSGIHSGATTTAPSLSGK) corresponding to the consensus GSK3 phosphorylation site of human β -Catenin (amino acids 29-49).
• Application: Immunoprecipitation: 5 μg of a previous lot immunoprecipitated β-Catenin from 500 μg of A431 membrane fraction lysate.Immunocytochemistry: 1-5 μg/mL of a previous lot showed positive immunostaining for β-catenin at cell-cell junctions of A431 cells fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton™ X-100.Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected beta-Catenin in A431 and HUVEC cell lines.
• Quality: Routinely evaluated by western blot on A431 total cell lysate.Western Blot Analysis: 0.1-1 μg/mL of this lot detected β-catenin in an A431 total cell lysate.
• Target description: 92 kDa
• Physical form: Format: Purified
• Storage and Stability: Stable for 1 year at 2-8°C from date of receipt.Handling Recommendations: Prior to removing the cap, centrifuge the vial and gently mix the solution.
• Analysis Note: ControlPositive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
• Legal Information: Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.