Anti-acetyl-Histone H3 (Lys9) Antibody

Description

• General description: Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
• Specificity: Histone H3 acetylated at position 9.
• Immunogen: Epitope: Surrounding H3K9ac
• Application: Anti-acetyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 acetylated on lysine 9. This purified Ab, also known as Anti-H3K9Ac, is specificity verified by dot blot (DB), published in peer reviewed journals, and validated in WB, ChIP, ChIP-seq, FC.
• Quality: Evaluated by Western Blotting in sodium butyrate treated HeLa cell lysate.Western Blotting Analysis: A 1:10,000 dilution of this antibody detected acetyl-Histone H3 (Lys9) in 10 µg of sodium butyrate treated HeLa cell lysate.
• Target description: ~17 kDa observed. Uncharacterized band(s) may appear in some lysates.
• Linkage: Replaces: 04-1003
• Physical form: Format: Purified
• Storage and Stability: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
• Legal Information: UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.