Anti-SNF2β/BRG1 Antibody

Description

• General description: The SNF2 family of proteins are ATP dependant chromatin remodeling enzymes. The gene encoding this protein is also refered to as SMARCA4.
• Specificity: This antibody recognizes the Near N-Terminus of SNF2B
• Immunogen: GST-tagged recombinant protein corresponding to the Near N-Terminus of human SNF2β.
• Application: Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected BRG1 in HeLa cells.
• Quality: Evaluated by Western Blotting in HELA NE cell lysate.Western Blotting Analysis: A 1:4,000 dilution of this antibody detected SNF2B/BRG1 in 10 µg of HELA NE cell lysate.
• Target description: 200 kDa
• Physical form: Rabbit polyclonal in buffer containing with 0.05% sodium azide with 30% glycerol.
• Storage and Stability: Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Analysis Note: ControlPositive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
• Legal Information: UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.