Anti-CTCF Antibody

Description

• General description: CTCF is a ubiquitous 11 zinc finger (ZF) protein with highly versatile functions. A transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator of APP. It is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. CTCF may also act as tumor suppressor. CTCF organizes epigenetically controlled chromatin insulators that regulate imprinted genes in soma.
• Specificity: Predicted to cross-react with mouse, rat, dog and primates based on sequence homology.
• Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 659-675 (C-TNQPKQNQPTAIIQVED) of human CCCTC-binding factor (CTCF) with a N-terminal cysteine added for conjugation purposes.
• Application: Chromatin Immunoprecipitation: An independent laboratory has confirmed that this antibody immunoprecipitates CTCF-bound chromatin from WEHI B-cells.
• Quality: Routinely evaluated by Western Blot.Western Blot Analysis: A 1:1000–1:5000 dilution of this lot detected CTCF in HeLa nuclear extract. A previous lot detected CTCF in K562 nuclear extract (data not shown).
• Target description: Approx. 120 kDa
• Linkage: Replaces: MABE547
• Physical form: Rabbit polyclonal antiserum containing 0.05% sodium azide and 30% glycerol.
• Storage and Stability: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
• Analysis Note: ControlPositive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 µL of extract and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced extract per lane for minigels.
• Other Notes: Concentration: The concentration of this serum antibody has not been determined.
• Legal Information: UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany