Mouse Anti-Human IgG₁, Fc Fragment Specific Alkaline Phosphatase Conjugate

Description

• General description: Protein A purified mouse monoclonal antibody conjugated to alkaline phosphatase. Recognizes human IgG1, Fc fragment.
• Application: ELISA (see comments)Enzyme Immunoassay (≥1:5000)Immunoelectrophoresis (see comments)
• Packaging: Please refer to vial label for lot-specific concentration.
• Warning: Toxicity: Standard Handling (A)
• Physical form: In 50 mM Tris, 1 mM MgCl₂, 50% glycerol, pH 8.0.
• Other Notes: Monospecific for human IgG1 Fc fragment as determined by immunoelectrophoresis and direct ELISA against normal human serum and normal human IgG. p-Nitrophenylphosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
• Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany