Goat Anti-Mouse IgG, H & L Chain β-Galactosidase Conjugate

Description

• General description: Immunoaffinity purified goat polyclonal antibody conjugated to β-galactosidase. Recognizes mouse IgG, heavy and light chains.
• Application: Enzyme Immunoassay (1:1000)Immunoelectrophoresis (see comments)
• Packaging: Please refer to vial label for lot-specific concentration.
• Warning: Toxicity: Standard Handling (A)
• Physical form: In 50 mM PBS, 10 mM glycine, 10 mM MgCl₂, 1% BSA, pH 7.0.
• Other Notes: To achieve maximum enzyme activity, we recommend using the following substrate: Dissolve 1 mg 4-methylumbelliferyl-β-D-galactoside (Cat. No. 474424) in 10 ml substrate buffer (10 mM sodium phosphate, pH 7.8, with 1 mM MgCl2) by stirring for 30 min at room temperature. Remove undissolved substrate by centrifuging or filtration. Monospecific for mouse IgG, heavy and light chains, as determined by immunoelectrophoresis against normal mouse serum. Variables associated with assay conditions will dictate the proper working dilution.
• Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany