Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate

Description

• General description: Purified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes human IgG Fc fragment.
• Application: Enzyme Immunoassay (1:1000, see application references)
• Packaging: Please refer to vial label for lot-specific concentration.
• Warning: Toxicity: Standard Handling (A)
• Physical form: In PBS, 10 mg/ml BSA.
• Other Notes: Antibody should be titrated for optimal results in individual systems.Cross-reactivity by ELISA against human myeloma proteins:Human IgG1: 100%Human IgG2: 100%Human IgG3: 100%Human IgG4: 100%Human IgM: <0.2%Human IgA: <0.1%Human IgE: <0.1%Cross-reactivity by ELISA against chimeric antibodies:Human IgG1: 100%Human IgG2: 100%Human IgG3: 100%Human IgG4: 100%Human IgM: <0.01%Human IgA: <0.01%Human IgE: <0.01%There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.
• Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany