A2306
Monoclonal Anti-Rabbit Immunoglobulins−Alkaline Phosphatase antibody produced in mouse
clone RG-16, purified immunoglobulin, buffered aqueous glycerol solution
Manufacturer: Sigma Aldrich
Synonym(S): Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 0.2 ML | A2306-0.2-ML | In Stock | ₹ 24,009.30 |
A2306 - 0.2 ML
In Stock
Quantity
1
Base Price: ₹ 24,009.30
GST (18%): ₹ 4,321.674
Total Price: ₹ 28,330.974
biological source
mouse
Quality Level
200
conjugate
alkaline phosphatase conjugate
antibody form
purified immunoglobulin
antibody product type
secondary antibodies
clone
RG-16, monoclonal
form
buffered aqueous glycerol solution
species reactivity
rabbit
should not react with
pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep
technique(s)
direct ELISA: 1:40,000immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160 using human tonsil and Cat. No. I8635 as the primary antibodywestern blot: 1:160,000-1:320,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per well)
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Description
- General description: Monoclonal Anti-Rabbit Immunoglobulins (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins is composed of two heavy (H) and two light (L) chains. Each containing one NH2-terminal variable region and one or more COOH-terminal constant regions. They are heterodimeric proteins.
- Specificity: Binds only rabbit Igs. Does not react with reduced rabbit Igs.
- Application: Monoclonal Anti-Rabbit Immunoglobulins Alkaline Phosphatase antibody produced in mouse has been used in: indirect enzyme linked immunosorbent assay (ELISA)immunoblottingimmunohistochemistry
- Biochem/physiol Actions: IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Mouse monoclonal anti-rabbit immunoglobulins-alkaline phosphatase antibody binds to an epitope on the heavy chain of rabbit IgG, IgA, and IgM. The product stains bands at the intact whole molecule and at the heavy chains in an immunoblot of denatured, non-reduced rabbit immunoglobulins. Reduction of rabbit immunoglobulins destroys the epitope. In ELISA, the product shows no cross reaction with human serum or tissue preparations, nor with human IgA, IgG, or IgM.
- Physical form: Solution in 0.05 M Tris buffer pH 8.0, containing 1 mM MgCl2, 0.1% bovine serum albumin, 50% glycerol and 15 mM sodium azide
- Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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biological source: mouse
Quality Level: 200
conjugate: alkaline phosphatase conjugate
antibody form: purified immunoglobulin
antibody product type: secondary antibodies
clone: RG-16, monoclonal
form: buffered aqueous glycerol solution
species reactivity: rabbit
should not react with: pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep
technique(s): direct ELISA: 1:40,000immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160 using human tonsil and Cat. No. I8635 as the primary antibodywestern blot: 1:160,000-1:320,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per well)
biological source:
mouse
Quality Level:
200
conjugate:
alkaline phosphatase conjugate
antibody form:
purified immunoglobulin
antibody product type:
secondary antibodies
clone:
RG-16, monoclonal
form:
buffered aqueous glycerol solution
species reactivity:
rabbit
should not react with:
pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep
technique(s):
direct ELISA: 1:40,000immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160 using human tonsil and Cat. No. I8635 as the primary antibodywestern blot: 1:160,000-1:320,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per well)
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biological source: synthetic
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synthetic
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powder
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