Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in rabbit

Description

• General description: IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids.IgG antibody is a glycoprotein, composed of two polypeptide chains (light and heavy), each possessing variable and constant domains. The variable region of IgG antibody is specific to antigens and is highly conserved. Anti-Mouse IgG (whole molecule)-Peroxidase antibody is specific for all mouse IgG subclasses. IgG is purified from mouse serum by fractionation and ion exchange chromatography. The purified IgG is then conjugated to peroxidase by protein cross-linking with 0.2% glutaraldehyde.
• Specificity: Anti-Mouse IgG (whole molecule)-Peroxidase antibody is specific for all mouse IgG subclasses.
• Immunogen: Purified mouse IgG
• Application: Anti-Mouse IgG (whole molecule)–Peroxidase antibody has been used in western blotting , immunohistochemistry and protein pin array assay.
• Biochem/physiol Actions: IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgG antibody has its function similar to IgM antibody in complement system activation.
• Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT
• Preparation Note: Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
• Storage and Stability: Store at -20 °C for long term. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots -20 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.