Anti-GluR2 Antibody

Description

• General description: Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses.
• Specificity: This antibody recognizes the cytoplasmic domain of Glutamate Receptor 2.
• Immunogen: Epitope: Cytoplasmic domain
• Application: Immunohistochemistry Analysis: A 1:4,000 dilution from a representative lot detected GluR2 in normal human and rat brain tissues.
• Quality: Evaluated by Western Blotting in mouse brain tissue lysate.Western Blotting Analysis: 0.5 µg/mL of this antibody detected GluR2 in 10 µg of mouse brain tissue lysate.
• Target description: ~108 kDa observed. Three isoforms at 99 kDa, 99 kDa, and 100 kDa are known to exist due to alternative splicing.There is no known homology to mouse and rat GluR1, GluR3 and GluR4. There was no homology detected to GluR1 and GluR3 in human, but there is 67% sequence homology to GluR4. An uncharacterized band may be observed at ~75 kDa in some cell lysates.
• Linkage: Replaces: AB1768-25UG
• Physical form: Affinity purified
• Storage and Stability: Stable for 1 year at 2-8°C from date of receipt.
• Analysis Note: ControlMouse brain tissue lysate
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.