Anti-phospho RasGRP2 (Ser116/Ser117)

Description

• General description: RAS guanyl-releasing protein 2 (UniProt: Q9QUG9; also known as Calcium and DAG-regulated guanine nucleotide exchange factor I, RasGRP2, CalDAG-GEFI, F25B3.3 kinase-like protein) is encoded by the Rasgrp2 gene (Gene ID: 19395) in murine species. RasGRP proteins are considered as activators of Ras and other related small GTPases by virtue o functioning as guanine nucleotide exchange factors (GEFs). RasGRP-1 through -4 share many structural domains, however, they display subtle differences between each of the different family members. They are expressed in distinct patterns, such as in different cells of the hematopoietic system and in the brain. RasGRP2′s expression is restricted to platelets and their precursors, megakaryocytes as well as neutrophils. RasGRP2 functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. It may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS, but not HRAS. It is also reported to functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils, probably through inside-out integrin activation. RaGRP2 is found both in the cytosol and associated with membranes. It is shown to be enriched at juxtamembrane areas and membrane ruffles. Three isoforms of RasGRP2 have been reported that are generated by alternative splicing. It contains two EF-hand domains, one N-terminal Ras-GEF domain, one phorbol-ester/DAG-type zinc finger domain, and one Ras-GEF domain. RasGRP2 deficient animals exhibit bleeding problems due to defects in platelets aggregation and degranulation. (Ref.: Olga Ksionda, O et al. (2013). Front Biol (Beijing). 8(5): 508 532).
• Specificity: This rabbit polyclonal antibody detects RAS guanyl-releasing protein 2 phosphorylated on Ser116/117 in Mouse and Monkey cells. It targets an epitope with in 12 amino acids surrounding Ser116/Ser117 from the N-terminal region.
• Immunogen: A linear peptide corresponding to 12 amino acids surrounding phosphorylated Ser116/117 from the N-terminal region of murine RAS guanyl-releasing protein 2.
• Application: Anti-phospho RasGRP2 (Ser116/Ser117), Cat. No. ABS2130, is a rabbit polyclonal antibody that detects RAS guanyl-releasing protein 2 phosphorylated on Ser116/Ser117 and has been tested for use in Immunofluorescence and Western Blotting.
• Quality: Evaluated by Western Blotting in RAS guanyl-releasing protein 2 purified from GST-RasGRP2 transfected COS-7 cells and phosphorylated on Ser116/Ser117 by GST-PKAc alpha.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RAS guanyl-releasing protein 2 in RasGRP2 purified from GST-RasGRP2 transfected COS-7 cells and phosphorylated on Ser116/Ser117 by GST-PKAc alpha
• Target description: ~90 kDa observed; 69.45 kda calculated. Uncharacterized bands may be observed in some lysate(s).
• Physical form: Affinity Purified
• Storage and Stability: Stable for 1 year at 2-8°C from date of receipt.
• Other Notes: Concentration: Please refer to lot specific datasheet.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.