Anti-Neurocan Antibody, N-terminal

Description

• General description: Neurocan core protein (UniProt: P55067; also known as 245 kDa early postnatal core glycoprotein, Chondroitin sulfate proteoglycan 3) is encoded by the Ncan (also known as Cspg3) gene in rat. Neurocan is a secreted protein of the aggrecan/versican proteoglycan family that is found in early postnatal and adult brain. During early postnatal development neurocan accounts for at least 20% of the protein of the soluble brain proteoglycans. Neurocan mostly contains chondroitin sulfate, but also has N-linked and O-linked oligosaccharides. It is shown to be involved in the modulation of cell adhesion and migration and regulates neurite outgrowth. Neurocan interact with several cell surface molecules, such as N-CAM, L1/Ng-CAM, etc., and modulates the cell-binding and neurite outgrowth promoting activates of these molecules. Neurocan is synthesized with a 22 aa signal peptide that is subsequently cleaved to produce the mature form. Neurocan contains one C-type lectin domain, two EGF-like domains, one Ig-like V type domain, two link domains and one Sushi (CCP/SCR) domain. (Ref.: Rauch, U., et al. (2001) Cell Mol. Life Sci. 58(12-13):1842-56; Zhou, XH et al. (2001). Mol. Cell Biol. 21(17): 5970-78).
• Immunogen: GST-tagged recombinant fragement corresponding to 164 amino acids from the N-terminal region of rat Neurocan.
• Application: Anti-Neurocan, N-terminal, Cat. No. ABT1384, is a rabbit polyclonal antibody that detects Neurocan core protein and has been tested for use in Immunofluorescence, Immunohistochemistry, and Radioimmunoassay.
• Quality: Evaluated by Immunofluorescence in E14 Embryonic rat brain (frozen section), rat E14 neocortex, and rat hippocampus cells.Immunofluorescence Analysis: A 1:500 dilution of this antibody detected Neurocan in E14 Embryonic rat brain (frozen section), rat E14 neocortex, and rat hippocampus cells.
• Target description: 135.55 kDa calculated.
• Physical form: Format: Unpurified
• Other Notes: Concentration: Please refer to lot specific datasheet.