Anti-Calbindin-D-28K (EG-20) antibody produced in rabbit

Description

• General description: Calbindin-D-28K (CALB1) is a calcium binding protein in the brain. This 27kDa protein is located on human chromosome 8q21.
• Immunogen: The immunogen sequence is specific for calbindin-D-28K and is not found in other members of the EF-hand family such as calbindin-D-9K, calretinin, myosin light chain, parvalbumin, S-100a, S-100b, S100A2 (S100L) and S100A6 (calcyclin). This sequence is highly conserved across species, including human, mouse, bovine, chicken, porcine, and frog calbindin-D-28K sequences.
• Application: Anti-Calbindin-D-28K (EG-20) antibody is suitable for use in immunohistochemistry (1:2,000 using sections of rat cerebellum), immunoblot and western blot (1:2,000 using a whole extract of rat brain). Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min., at RT or 37 ° C) improves immunohistochemical staining with the antibody.Anti-Calbindin-D-28K (EG-20) antibody has been used in immunohistochemical (IHC) peroxidase staining and cupric silver staining. It has also been used in immunofluorescence.
• Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
• Analysis Note: Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min at RT or 37 °C) improves immunohistochemical staining.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.