Monoclonal Anti-CD8 antibody produced in mouse

Description

• General description: Monoclonal Anti-Human CD8 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from BALB/c mice immunized with human thymocytes followed by peripheral blood T cells. CD8 (cluster of differentiation 8), is a dimeric protein, encoded by the CD8A gene mapped to human chromosome 2p12.
• Specificity: Recognizes the CD8 human T cytotoxic/suppressor lymphocyte cell surface glycoprotein. Approximately 90% of thymocytes are stained in suspension. In thymus sections, both cortical and medullary thymocytes are stained. A subset of NK cells express this antigen weakly. The epitope recognized by this clone is sensitive to routine formalin fixation and paraffin embedding.3rd Workshop: code no. 567
• Immunogen: human thymocytes followed by peripheral blood T cells.
• Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Flow cytometry/Cell sorting (1 paper)Immunofluorescence (1 paper)
• Biochem/physiol Actions: CD8 might serve as a marker for neoplastic diseases and acute myeloid leukemia.
• Target description: CD8 is a two chain complex (α/α, α/β) receptor for Class I MHC molecules. The receptor′s cytoplasmic portion is associated with p56lck protein tyrosine kinase which phosphorylates nearby proteins. The antigen is strongly expressed on approximately one third of mature T cells (cytotoxic/suppressor T cells).
• Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
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