Anti-CD4−FITC antibody, Mouse monoclonal

Description

• General description: Anti-CD4-FITC antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from Balb/c mice immunized with cluster of differentiation 4 (CD4)-transfected mouse T cell hybridoma, 3DT, followed by CD4+ human T cell CEM cells (leukemic lymphoblasts).
• Specificity: Recognizes the CD4. The epitope recognized by the Q4120 clone is located on 1-183 aa and is sensitive to formalin fixation and paraffin embedding. 5th Workshop: code no. CD04.11
• Immunogen: CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.
• Application: FITC Conjugated Monoclonal Anti-Human CD4 has been used for:identification, quantification and monitoring of helper/inducer T cells in peripheral blood, biological fluids, lymphoid organs, and other tissuesanalysis of T cell mediated cytotoxicitycharacterization of subtypes of T cell leukemias and lymphomasstudies of T cells in health and diseaseflow cytometry
• Biochem/physiol Actions: CD4+ T cells acts as a helper/inducer, in providing an activating signal to B cells. It induces T lymphocytes bearing the reciprocal CD8 marker to become cytotoxic/suppressor cells. It is also majorly associated with non-polymorphic region of MHC (major histocompatibility complex) antigen in interacting with targets bearing MHC class II molecules. Thus, it is called as an adhesion molecule. It also behaves as receptor for the GP 120 envelope glycoprotein of human immunodeficiency virus. The CD4 molecules is involved in the adhesion of T lymphocytes to target cells, thymic development, transmission of intracellular signals during T cell activation, and binding to polyclonal immunoglobulins.
• Target description: CD4 is a single chain transmembraneous glycoprotein from the immunoglobulin superfamily. It is expressed on the helper/inducer T subset, on most medullary thymocytes, on microglial, dendritic and on some malignancies of T cell origin. The antigen binds to MHC class II molecules and is associated with p56lck protein tyrosine kinase.
• Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
• Preparation Note: Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.