Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody produced in goat

Description

• General description: IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids.Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.
• Specificity: Goat polyclonal anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody isolated from goat antiserum by affinity chromatography reacts specifically with mouse Fc fragment. No cross reaction is observed with mouse Fab fragment. The antibodies are adsorbed to assure minimal cross reaction with human, horse and bovine proteins on tissue or cell preparations.
• Immunogen: Purified Fc fragments of mouse IgG
• Application: Anti-Mouse IgG (Fc specific) F(ab′)2 fragment-FITC antibody produced in goat has been used in : flow cytometry for labelling of human peripheral blood lymphocytes (dilution of 1:100) and label human polymorphonuclear cells (PMNs) (dilution of 1:50).immunocytochemistry using human embryonic stem cellsimmunohistochemistry at a working dilution of 1:160.
• Biochem/physiol Actions: immunoglobulin G (IgG) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG antibody participates in complement fixation and opsonization.
• Other Notes: Antibody adsorbed with bovine, equine and human serum proteins.
• Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
• Preparation Note: Adsorbed to reduce background with bovine, horse or human samples.Useful when trying to avoid background staining due to the presence of Fc receptors.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.