Anti-Histone Deacetylase 4 (HDAC4) (DM-15) antibody produced in rabbit

Description

• General description: Histone deacetylases (HDACs) are competing enzymes, belonging to histone deacetylase family. There are two classes of HDACs with six to seven different types of HDACs proteins. HDAC1,HDAC2 and HDAC3 belong to Class I HDACs and HDAC4, HDAC6, and HDAC7 belong to Class II HDACs. Class I HDACs consists of a single deacetylase domain at the N-termini and diversified C-terminal regions, while Class II contains a deacetylase domain at C-terminal position.
• Specificity: Anti-Histone Deacetylase 4 (HDAC4), recognizes human, mouse and rat HDAC4.
• Immunogen: Synthetic peptide corresponding to amino acid residues of human HDAC4 with C-terminal added lysine conjugated to KLH.
• Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below:Western BlottingImmnofluorescenceImmunoprecipitation
• Biochem/physiol Actions: Histone deacetylase 4 (HDAC4) contains import and export nuclear signal that aids in nucleocytoplasmic shuttling. It has physical and functional interactions with transcription factor myocyte enhancer factor-2 (Mef2) protein and 14-3-3. This interaction disturbs nucleocytoplasmic shuttling and then guides HDAC4 to the cytoplasm and the nucleus, respectively. In addition, HDAC4 with HDAC5 and HDAC7 represses transcription by binding to the nuclear receptor co-repressors silencing mediator of retinoic acid and thyroid hormone receptors (SMRT) and nuclear receptor co-repressor (NCOR).
• Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
• Storage and Stability: For continuous use, store at 2-8°C for up to one month. For prolonged storage, freeze in working aliquots at -20°C. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.