Anti-MCAM Antibody, clone P1H12

Description

• General description: MUC18 antigen (CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MUC18 has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MUC18. The close structural relationship with N-CAM and related molecules suggests that MUC18 may be also a developmentally regulated cell adhesion molecule (Melanoma adhesion molecule or MCAM).
• Specificity: Detects circulating endothelial cells in human. Expected to cross-react with dog & mouse.
• Immunogen: Immunization of mice with HUVEC.
• Application: Anti-MCAM Antibody, clone P1H12 detects level of MCAM & has been published & validated for use in ELISA, FC, IC, IH, IP & WB.
• Quality: Routinely evaluated by Western Blot on HUVEC lysates.Western Blot Analysis: 1:500 dilution of this lot detected MCAM on 10μg of HUVEC lysates.
• Target description: 110 kDa
• Physical form: Format: Purified
• Analysis Note: ControlHUVEC cells.
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
• Legal Information: CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany