Anti-Integrin α1 Antibody, clone FB12

Description

• Specificity: Reacts with the I domain (Val151 - Ala364) of human alpha-1 integrin (CD49a)1. Precipitates proteins of Mr 180 kDa, pI 5.9-6.0 (alpha chain) and Mr 115 kDa, pI 4.5-5.0 (beta chain) from human lymphocyte lysates (Zocchi et al., 1991).
• Application: Immunohistochemistry: 2 μg/mL, with paraformaldehyde fixation.Immunohistochemistry: Frozen sections (7 µm) of placental tissue fixed with acetone for 1 hr. Antibody used at 1:10 dilution (Sato et al., 2003).Immunocytochemistry: iolated EVT cells firxed with 0.5% PFA @ 4°C for 15 minutes followed by acetone at -20°C for 2 minutes. Antibody was used at 1:10 dilution (Sato et al., 2003).Immunoblotting: 2 μg/mL Immunoprecipitation: 1 μg/mL FACS analysis: 2μg/mL (Sato et al., 2005).ELISA: 0.5 μg/mL Inhibition of activated human lymphocyte binding to laminin, collagen IV and fibronectin (Fabbri et al., 1996): 1-5 μg/mL Optimal working dilutions must be determined by end user.
• Target description: 130 kDa
• Physical form: Format: Purified
• Analysis Note: ControlMicrovascular endothelium
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
• Legal Information: CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany