Anti-Integrin αVβ3 Antibody, clone LM609, Phycoerythrin conjugated

Description

• General description: EMISSION MAXIMUM: 580 nm
• Specificity: Monoclonal antibody LM609 is reactive with the vitronectin receptor alphaVbeta3 complex, an RGD-directed adhesion receptor. LM609 has been demonstrated to block adhesion of a human melanoma cell line (M21) to vitronectin, fibrinogen and von Willebrand factor, as well as to a synthetic RGD containing peptide (Cheresh, 1987). In chick chorioallentoic membranes, LM609 was shown to block angiogenesis induced by bFGF and TNFalpha but had no effect on pre-existing vessels (Brooks, 1994). While LM609 does block cell attachment to RGD containing ligands, it does not interact directly with the RGD binding site. Instead, LM609 appears to be an allosteric inhibitor of integrin alphaVbeta3, which binds to a conformational epitope resulting from the post-translational association of the alphaV and beta3 subunits. ABSORPTION MAXIMA: 90 nm, 545 nm and 565 nm
• Immunogen: Purified adhesion receptor from the human melanoma cell line M21 (Cheresh, 1987).
• Application: Anti-Integrin αVβ3 Antibody, clone LM609, Phycoerythrin conjugated detects level of Integrin αVβ3 & has been published & validated for use in FC, IH.
• Physical form: Liquid in 0.01M sodium phosphate (pH7.6), 0.25 M NaCl, 0.05% sodium azide, 0.01% Thimerosal and 15 mg/mL BSA added as a stabilizer.
• Storage and Stability: Maintain at 2-8°C in undiluted aliquots for up to 6 months. Protect from Light. Do not store as a dilute solution.
• Analysis Note: ControlPOSITIVE CONTROL: Human myeloma cell lines M21 and MoalphaV (Chen, 1995). NEGATIVE CONTROL: Mo cells (this cell line was derived from M21 but does not express integrin alphaV; Chen, 1995).
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
• Legal Information: CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.