Anti-Integrin αVβ6 Antibody, clone E7P6, azide free

Description

• Specificity: Monoclonal antibody MAB2074Z, raised by immunizing Balb/c mice with recombinant secreted human avb6, is useful for flow cytometry and is especially useful for immunocytochemistry. The antibody works well for immunohistochemistry of human tissue, especially under circumstances where upregulation of the receptor is being evaluated. The antibody does not stain formalin-fixed or paraffin-embedded tissues, and may not detect the low-level basal expression of alphavbeta6 in epithelia of healthy adults. This antibody does not recognize the alphaV subunit and does not react with alphaV integrins other than alphavbeta6. Because the antibody is not effective in western blot, it is unknown whether the epitope recognized by the antibody localizes to the alphavbeta6 heterodimer or to the beta6 subunit only.
• Immunogen: Recombinant secreted human alphavbeta6.
• Application: Immunohistochemistry (acetone fixation): Effective for human tissue, especially in circumstances where up-regulation of the receptor is being evaluated. The antibody does not stain formalin-fixed or paraffin-embedded tissues, and may not detect the low-level basal expression of alpavbeta6 in epithelia of healthy adults. Immunocytochemistry: Effective for F6, HT-29, H441 cell lines (others not tested). Flow cytometry: Effective for detection of human and monkey alphaVbeta6 Not effective for immunoprecipitation or western blot. Optimal working dilutions must be determined by end user.
• Physical form: Format: Purified
• Storage and Stability: Maintain at 2-8°C in undiluted aliquots for up to 12 months.
• Legal Information: CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.