Anti-Cell Surface Antigen of Human Nucleated Erythroid Cells Antibody, clone HAE9

Description

• Specificity: MAB2115 recognizes a 70 kDa surface protein in about 100% nucleated erythroid cells (erythroblasts, normoblasts, proerythroblasts), 36% of late erythroid committed cells and 70-80% of fetal liver cells. Normal stem cells, early erythroid committed cells and hematopoetic non-erythroid cells do not react with MAB2115. The 70 kDa protein may be associated with an isoform of transferrin specific for red cells. Applicable for (1) immunostaining and enumeration of nucleated erythroid cells in bone marrow, fetal liver or cord blood and (2) immunophenotyping human leukemias. {Mechetner, EB et al (1987) Identification of a human erythroid cell surface antigen by monoclonal antibody HAE9. Exp Hematol. 15(4):355-9}.
• Immunogen: Human fetal liver cells
• Application: Immunoprecipitation: 5μg/500μL of cell lysate in 0.5% triton X-100 diluted in PBS with proteinase inhibitors Flow cytometry: 10μg/mL concentration; (1:100); per 10(e6) cells, 100μL sample volume. Optimal working dilutions must be determined by the end user.
• Physical form: Format: Purified
• Storage and Stability: Maintain at 2-8°C for up to 6 months after date of receipt. The product may contain precipitation and microcentrifugation is recommended before use.
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
• Legal Information: CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.