Anti-A2B5 Antibody, clone A2B5-105

Description

• General description: A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors and neuroendocrine cells. The A2B5 antigen is present in type 2 astrocytes but absent in type 1 astrocytes, allowing for the differentiation of these different types of cells. A2B5 antibody has been used in flow cytometry and immunohistochemistry experiments to stain type II astrocytes, cells involved in gliogenesis and cells committed to the oligodendrocyte lineage.
• Specificity: Recognizes an epitope common to silaogangliosides and sulfatides from the plasma membranes of neurons, and neuroendocrine and glial cells. Anti-A2B5 has been used to specifically stain: Neurofibrillary tangles in the post-mortem brain tissue of patients with a confirmed diagnosis of Alzheimer′s disease (Majocha et al., 1989) Neurons of chicken retina, brain, spinal cord, and dorsal root ganglia (Schnitzer & Schachner, 1982; Eisenbarth et al., 1979). Rat striatal nerve terminals [synaptosomes (Wolf & Kapatos, 1989)]. Neuroblastoma cells in man (Oppedal et al., 1989; Coakham et al., 1985). Rat insulinoma cells and pancreatic islets cells (Shimizu et al., 1983; Bartholomeusz et al., 1989). Human and rat thymic epithelial cells (Haynes et al., 1988). Cells involved in gliogenesis (Bottenstein et al., 1988; Suzumura & Slerberg, 1989). Oligodendrocytes and astrocytes, type II (Dubois-Dalcq et al., 1990).
• Immunogen: Embryonic chicken retinal cells
• Application: Anti-A2B5 Antibody, clone A2B5-105 detects level of A2B5 & has been published & validated for use in FC, IC, IF, IH.
• Physical form: Ammonium Sulfate Precipitation
• Storage and Stability: Maintain the lyophilizate frozen at -20°C. Store the reconstituted antibody solution at -20°C for 12 months in undiluted aliquots.
• Analysis Note: ControlType II astrocytes, human neural progenitors
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
• Legal Information: CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.