Anti-5-methylcytosine Antibody, clone 33D3

Description

• General description: 5-Methylcytosine is a modified base found in the DNA of higher eukaryotes. Methylation of DNA is known to be involved in the control of gene expression and is mediated by a class of enzymes known as DNA methyltransferases (DNMT’s). Appropriate cytosine methylation is essential for normal development and maintenance of mammalian cells. Alternations in DNA methylation patterns is often associated with cancers with the turmor DNA being hypomethylated compared to DNA from normal tissues.
• Specificity: Predicted to react with multiple species.
• Immunogen: Ovalbumin-conjugated 5-methylcytosine
• Application: IFHabib, M., et al. (1999). Exp Cell Res. 249:46-53 IHC-PHernadez-Blazquez, F.J., et al. (2000). Gut. 47:689-693 Flow CytometryReynaud, C.C., et al. (1992). Cancer Lett. 61(3):255-262MeDIPKeshet, I., et al. (2006). Nat Genet. 38(2):149-153
• Quality: Evaluated by Dot Blot in methylated and unmethylated BRCA1.Dot Blot Analysis: 1 µg/mL of this antibody detected methylcytosine in methylated and unmethylated BRCA1.
• Target description: Detects methylated cytosine
• Linkage: Replaces: MABE527
• Physical form: Format: Purified
• Storage and Stability: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Analysis Note: ControlMethylated and unmethylated BRCA1
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.