Anti-IL-4 (mouse), APC, clone 11B11 Antibody

Description

• General description: The 11B11 antibody binds to mouse Interleukin-4 (IL-4), a 14 kDa cytokine that is largely secreted by activated T cells of the Th2 subset, and to some degree by NKT and mast cells. This cytokine acts as a stimulatory factor for B cells, inducing their proliferation and differentiation, as well as playing a role in immunoglobulin class-switching. IL-4 may also provide autocrine stimulation for T cells, and affect the function of antigen presenting cells such as macrophages and dendritic cells. IL-4 can bind and signal via three cell surface receptor types: CD124 by itself, CD124 in combination with the common gamma chain (type I complex), or CD124 combined with CD213a1 (type II complex).The 11B11 antibody is widely used for detection of intracellular levels of IL-4 protein by flow cytometry, as well as for analysis of soluble cytokine as measured by ELISA, and in functional assays to neutralization cytokine-receptor interactions.
• Immunogen: Immunogen information for this antibody is proprietary information.
• Application: Research CategoryInflammation & Immunology
• Quality: This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.Flow Cytometry Analysis: 0.5 μg from a representative lot detected IL-4 in one million C57Bl/6 splenocytes.
• Physical form: Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
• Storage and Stability: Stable for 1 year at 2-8°C from date of receipt.Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
• Other Notes: Concentration: Please refer to lot specific datasheet.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.