Anti-Complement Receptor C5aR Antibody, clone 20/70

Description

• General description: C5a anaphylatoxin chemotactic receptor 1 (UniProt: P30993; also known as C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, CD88) is encoded by the C5ar1 (also known as C5ar, C5r1) gene (Gene ID: 12273) in murine species. C5aR is a multi-pass membrane protein of the G-protein coupled receptor 1 family that serves as a receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production. C5aR is a homodimer, but can also form higher-order oligomers. C5aR becomes phosphorylated on serine residues in response to C5a binding that results in internalization of the receptor and short-term desensitization to C5a. The phosphorylated receptor is known to Interact with arrestin beta 1 and 2 (ARRB1 and 2) and this interaction is associated with internalization of C5aR. Sulfation is also reported to play a critical role in the association of C5aR with C5a, but it does not alter the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.
• Specificity: Clone 20/70 detects complement 5a receptor in murine species. It targets an epitope with in the extracellular domain.
• Immunogen: Rat basophilic leukemia RBL-2H3 cells stably expressing murine C5aR.
• Application: Anti-Complement Receptor C5aR, clone 20/70, Cat. No. MABF1976, is a rat monoclonal antibody that detects Complement 5a receptor (C5aR) and has been tested for use in Flow Cytometry, Activity Assay, Inhibition of Activity/Function, Immunocytochemistry, and Neutralizing.
• Quality: Evaluated by Flow Cytometry in BALB/c mouse bone marrow cells.Flow Cytometry Analysis: 1 µg of this antibody detected Complement receptor C5aR in 1 million BALB/c mouse bone marrow cells.
• Target description: 39.02 kDa calculated.
• Physical form: Format: Purified
• Other Notes: Concentration: Please refer to lot specific datasheet.