Anti-CD117 (c-Kit) Antibody, clone 2C11

Description

• General description: Mast/stem cell growth factor receptor Kit (UniProt: P10721; also known as EC: 2.7.10.1, SCFR, Piebald trait protein, PBT, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog) is encoded by the KIT (also known as SCFR) gene (Gene ID: 3815) in human. C-Kit is a single-pass type I membrane protein with tyrosine protein kinase activity and serves as a cell-surface receptor for the cytokine KITLG/SCF and is shown to play a significant role in the regulation of cell survival, cell proliferation, stem cell maintenance, gametogenesis and other functions. c-Kit exists as a monomer in the absence of bound KITLG/SCF, however, it homodimerizes in the presence of bound KITLG/SCF and forms a heterotetramer with two KITLG/SCF molecules. c-Kit can be autophosphorylated on tyrosine residues and KITLG/SCF binding enhances its autophosphorylation. Its kinase activity is down-regulated by phosphorylation on serine residues by PKC family members. Phosphorylation at Tyrosine 568 is essential for its interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. c-Kit can be ubiquitinated by SOCS6 and it is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to its internalization and degradation. c-Kit is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to produce the mature form with an extracellular domain (aa 26-524), a short transmembrane domain (aa 525-545), and a cytoplasmic domain (aa 546-976. Its two ATP-binding sites are localized to amino acids 596-603 and 671-677. Lack of c-Kit expression has been reported in multiple cancer types and c-Kit expressing cells display a regression of typical morphological features of malignancy.
• Specificity: Clone 2C11 specifically detects CD117 (c-Kit) in human and murine cells. It targets an epitope with in the extracellular domain.
• Immunogen: GST-tagged recombinant fragment corresponding to 195 amino acids from the extracellular domain of Human C-kit.
• Application: Flow Cytometry Analysis: A representative lot detected CD117 (c-Kit) in P815 cells (Courtesy of the Monoclonal Antibody Unit at the Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore).Western Blotting Analysis: A representative lot detected CD117 (c-Kit) in DLD-1 cell lysate (Courtesy of the Monoclonal Antibody Unit at the Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore).
• Quality: Evaluated by Western Blotting in DLD-1 cell lysate.Western Blotting Analysis: 1 µg/mL of this antibody detected CD117 (c-Kit) in DLD-1 cell lysate.
• Target description: ~160 kDa observed; 109.87 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
• Physical form: Format: Purified
• Other Notes: Concentration: Please refer to lot specific datasheet.