Anti-CD8 alpha Antibody, clone 53-6.72

Description

• General description: T-cell surface glycoprotein CD8 alpha chain (UniProt: P01731; also known as T-cell surface glycoprotein Lyt-2, CD8a) is encoded by the CD8a (also known as Lyt-2, Lyt2) gene (Gene ID: 12525) in murine species. CD8 alpha is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-27), which is subsequently cleaved to form the mature protein that has an extracellular domain (aa 28-196), a helical domain (aa 197-217), and a cytoplasmic tail (aa 218-247). CD8 is generally present as a heterodimer of alpha and beta chains linked by two disulfide bonds, however, it can also form homodimers. The CD8 alpha/alpha homodimer is expressed on a subset of gamma/delta TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 plays a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. CD8 acts as a co-receptor for the T cell receptor (TCR) and like the TCR, it binds to class I MHC molecules displayed by antigen presenting cells (APC). CD8 is primarily expressed on the surface of cytotoxic T cells, but can also be found on thymocytes, natural killer cells, and some dendritic cell subsets.
• Specificity: Clone 53-6.72 is a rat monoclonal antibody that detects CD8 alpha chain in murine thymocytes.
• Immunogen: Murine Thymic/splenic lymphocytes.
• Application: Flow Cytometry Analysis: A representative lot detected CD8 alpha in Flow Cytometry applications (de Latour, R.P., et. al. (2010). Blood. 116(20):4175-84).Functional Analysis: A representative lot detected CD8 alpha in Functional analysis applications (Daley, D., et. al. (2016). Cell. 166(6):1485-1499; Hannani, D., et. al. (2014). Cell Death Differ. 21(1):50-8).
• Quality: Evaluated by Flow Cytometry in mouse thymocytes.Flow Cytometry Analysis: 1 µg of this antibody detected CD8 alpha in one million mouse thymocytes.
• Target description: 27.46 kDa calculated.
• Physical form: Format: Purified
• Other Notes: Concentration: Please refer to lot specific datasheet.